期刊
MICROBIAL BIOTECHNOLOGY
卷 14, 期 2, 页码 403-418出版社
WILEY
DOI: 10.1111/1751-7915.13594
关键词
-
资金
- Fundamental Research Funds for the Central Universities [DUT20GJ217]
- National Natural Science Founfation of China [31701719]
- LiaoNing Revitalization Talents Program [XLYC1907085]
The study identified the bacteriophage D2 with potent antimicrobial activity against multidrug-resistant bacteria, particularly Acinetobacter baumannii, and its endolysin Abtn-4 showed the ability to reduce biofilm formation. Abtn-4 exhibited broad-spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria, as well as phage-resistant bacterial mutants. These findings suggest that endolysin Abtn-4 may serve as a promising candidate for the treatment of multidrug-resistant bacterial infections.
The emergence and rapid spread of multidrug-resistant bacteria has induced intense research for novel therapeutic approaches. In this study, theAcinetobacter baumanniibacteriophage D2 (vB_AbaP_D2) was isolated, characterized and sequenced. The endolysin of bacteriophage D2, namely Abtn-4, contains an amphipathic helix and was found to have activity against multidrug-resistant Gram-negative strains. By more than 3 log units,A. baumanniiwere killed by Abtn-4 (5 mu M) in 2 h. In absence of outer membrane permeabilizers, Abtn-4 exhibited broad antimicrobial activity against several Gram-positive and Gram-negative bacteria, such asStaphylococcus aureus,Pseudomonas aeruginosa,Klebsiella pneumonia,EnterococcusandSalmonella. Furthermore, Abtn-4 had the ability to reduce biofilm formation. Interestingly, Abtn-4 showed antimicrobial activity against phage-resistant bacterial mutants. Based on these results, endolysin Abtn-4 may be a promising candidate therapeutic agent for multidrug-resistant bacterial infections.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据