期刊
CELLS
卷 9, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/cells9081806
关键词
intron circle; intronic circRNA; exonic circRNA; intron; non-coding; intronic lariat; sisRNA; circular junction; sub-exonic circRNA; pig testis
类别
资金
- Animal Genetics Division of INRAE as part of the PigTRome project
The sequencing of total RNA depleted for ribosomal sequences remains the method of choice for the study of circRNAs. Our objective was to characterize non-canonical circRNAs, namely not originating from back splicing and circRNA produced by non-coding genes. To this end, we analyzed a dataset from porcine testis known to contain about 100 intron-derived circRNAs. Labelling reads containing a circular junction and originating from back splicing provided information on the very small contribution of long non-coding genes to the production of canonical circRNAs. Analyses of the other reads revealed two origins for non-canonical circRNAs: (1) Intronic sequences for lariat-derived intronic circRNAs and intron circles, (2) Mono-exonic genes (mostly non-coding) for either a new type of circRNA (including only part of the exon: sub-exonic circRNAs) or, even more rarely, mono-exonic canonical circRNAs. The most complex set of sub-exonic circRNAs was produced byRNase_MRP(ribozyme RNA). We specifically investigated the intronic circRNA ofATXN2L, which is probably an independently transcribed sisRNA (stable intronic sequence RNA). We may be witnessing the emergence of a new non-coding gene in the porcine genome. Our results are evidence that most non-canonical circRNAs originate from non-coding sequences.
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