期刊
JOURNAL OF CLINICAL MEDICINE
卷 9, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/jcm9061882
关键词
gastric microbiota; transient; persistent; culture; microbiome; sequencing; Helicobacter pylori
资金
- Lithuanian Research Council [APP-2/2016]
Helicobacter pyloriis a common colonizer of the human stomach, and long-term colonization has been related to development of atrophic gastritis, peptic ulcers and gastric cancer. The increased gastric pH caused byH. pyloricolonization, treatment with antibiotics or proton pump inhibitors (PPI) may allow growth of other bacteria. Previous studies have detected non-Helicobacterbacteria in stomach biopsies, but no conclusion has been made of whether these represent a transient contamination or a persistent microbiota. The aim of this study was to evaluate the transient and persistent bacterial communities of gastric biopsies. The washed or unwashed gastric biopsies were investigated by cultivation and microbiota analysis (16S rRNA gene-targeted amplicon sequencing) for the distribution ofH. pyloriand other non-Helicobacterbacteria. The number of cultured non-Helicobacterbacteria decreased in the washed biopsies, suggesting that they might be a transient contamination. No significant differences in the bacterial diversity were observed in the microbiome analysis between unwashed and washed biopsies. However, the bacterial diversity in biopsies shownH. pylori-positive andH. pylori-negative were significantly different, implying thatH. pyloriis the major modulator of the gastric microbiome. Further large-scale studies are required to investigate the transient and persistent gastric microbiota.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据