期刊
EBIOMEDICINE
卷 57, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ebiom.2020.102885
关键词
SARS-CoV-2; COVID-19; Severity; Outcome; T cells; T cell memory; T cell response; Flow cytometry; GLP; Standardization
资金
- State of Lower Saxony [14-76103-184 CORONA-11/20]
- German Research Foundation, Excellence Strategy [EXC 2155, ID39087428]
- DFG [SFB900/3, ID158989968, SFB900-B8, SFB900-B3]
Background: Elucidating the role of T cell responses in COVID-19 is of utmost importance to understand the clearance of SARS-CoV-2 infection. Methods: 30 hospitalized COVID-19 patients and 60 age-and gender-matched healthy controls (HC) participated in this study. We used two comprehensive 11-colour flow cytometric panels conforming to Good Laboratory Practice and approved for clinical diagnostics. Findings: Absolute numbers of lymphocyte subsets were differentially decreased in COVID-19 patients according to clinical severity. In severe disease (SD) patients, all lymphocyte subsets were reduced, whilst in mild disease (MD) NK, NKT and gamma delta T cells were at the level of HC. Additionally, we provide evidence of T cell activation in MD but not SD, when compared to HC. Follow up samples revealed a marked increase in effector T cells and memory subsets in convalescing but not in non-convalescing patients. Interpretation: Our data suggest that activation and expansion of innate and adaptive lymphocytes play a major role in COVID-19. Additionally, recovery is associatedwith formation of T cellmemory as suggested by themissing formation of effector and central memory T cells in SD but not in MD. Understanding T cell-responses in the context of clinical severity might serve as foundation to overcome the lack of effective anti-viral immune response in severely affected COVID-19 patients and can offer prognostic value as biomarker for disease outcome and control.
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