期刊
SCIENCE ADVANCES
卷 6, 期 26, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aaz0495
关键词
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资金
- Burroughs Wellcome Fund Career Award at the Scientific Interface (CASI)
- Stanley Fahn PDF Junior Faculty Grant [PF-JFA-1760]
- Beckman Foundation Young Investigator Award
- USDA AFRI award
- USDA NIFA award
- Moore Foundation
- FFAR New Innovator Award
- Schlumberger Foundation Faculty for the Future Fellowship
- University of California Berkeley Molecular Imaging Center
- QB3 Shared Stem Cell Facility
- Innovative Genomics Institute (IGI)
Posttranscriptional gene silencing (PIGS) is a powerful tool to understand and control plant metabolic pathways, which is central to plant biotechnology. PIGS is commonly accomplished through delivery of small interfering RNA (siRNA) into cells. Standard plant siRNA delivery methods (Agrobacterium and viruses) involve coding siRNA into DNA vectors and are only tractable for certain plant species. Here, we develop a nanotube-based platform for direct delivery of siRNA and show high silencing efficiency in intact plant cells. We demonstrate that nanotubes successfully deliver siRNA and silence endogenous genes, owing to effective intracellular delivery and nanotube-induced protection of siRNA from nuclease degradation. This study establishes that nanotubes could enable a myriad of plant biotechnology applications that rely on RNA delivery to intact cells.
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