期刊
ACS SENSORS
卷 5, 期 6, 页码 1822-1830出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.0c00694
关键词
electrolyte-gated organic thin-film transistor; single molecule with a large transistor (SiMoT); genomic markers; biosensors; early diagnosis; DNA detection; miRNAs; multiple sclerosis
资金
- OrgBIO Organic Bioelectronics [PITN-GA-2013-607896]
- PON SISTEMA (MIUR)
- H2020-Electronic Smart Systems-SiMBiT: Single molecule bio-electronic smart system array for clinical testing [824946]
- Future in Research APQ Ricerca Regione Puglia Programma regionale a sostegno della specializzazione intelligente e della sostenibilita sociale ed ambientale-FutureInResearch-BEND Biosensori elettronici intelligenti per la diagnosi precoce di malattie n [B164PG8]
- PMGB-Sviluppo di piattaforme meccatroniche, genomiche e bioinformatiche per l'oncologia di precisione RICERCA E INNOVAZIONE 2014-2020 projects [ARS01_01195-PON]
- CSGI
The increasing interest in technologies capable of tracking a biomarker down to the physical limit points toward new opportunities in early diagnostics of progressive diseases. Indeed, single-molecule detection technologies are foreseen to enable clinicians to associate the tiniest increase in a biomarker with the progression of a disease, particularly at its early stage. Bioelectronic organic transistors represent an extremely powerful tool to achieve label-free and single-molecule detection of clinically relevant biomarkers. These electronic devices are millimetric in size and in the future could be mass-produced at low cost. The core of the single molecule with a large transistor (SiMoT) platform, based on an electrolyte-gated field-effect transistor, is a gold gate electrode biofunctionalized with a self-assembled monolayer, a densely packed layer of recognition elements. So far, only the SiMoT detection of proteins, using the corresponding antibodies as recognition elements, has been reported. In this study, the SiMoT sensing response toward genomic biomarkers is proposed. Herein, the gate is functionalized with a genomic biomarker for multiple sclerosis (miR-182). This is relevant, not only because a limit of detection of a single molecule is achieved but also because it proves that the SiMoT label-free, single-molecule detection principle is the only one of its kind that can detect, by means of the same platform, both protein and genomic markers.
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