期刊
FRONTIERS IN CHEMISTRY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2020.00654
关键词
sodium alginate; platinum nanozyme; oxidase-like activity; antioxidant capacity; colorimetric; proanthocyanidins
资金
- National Natural Science Foundation of China [21675024]
- Program for Innovative Leading Talents in Fujian Province [2016B016]
- Program for Innovative Research Team in Science and Technology in Fujian Province University [2018B033]
- Science and Technology Project of Fujian Province [2018L3008]
Platinum nanozymes exhibiting highly efficient and robust oxidase-like activity are successfully synthesized and modified using sodium alginate (SA-PtNPs). According to a steady-state dynamic assay, Michaelis-Menton constant (K-m) is calculated as 11.6 mu M, indicating that the affinity of SA-PtNPs toward the substrate, 3, 3 ', 5, 5 '-tetramethylbenzidine (TMB), is high. It shows in the paper that SA-PtNPs exhibit a significant oxidant effect on substrate-O(2)to produceO2 center dot-as an oxidase mimic. Moreover, the oxidase-like activity fluctuated slightly under changes in environmental pH and incubation time, implying that SA can increase the dispersibility and stability of PtNPs. A colorimetric assay for oligomeric proanthocyanidins (OPC) was realized given how few explorations of the former there are. We found that the significant inhibitory effect of OPC on the oxidase-like activity is due to the competitive effect between OPC and TMB for binding to the active site of SA-PtNPs, resulting in a color change. Under optimal conditions, the logarithmic value of the chromogenic difference (Delta A(450nm)) to OPC concentration was linear (4-32.5 mu M,r= 0.999) with a limit of detection (LOD) of 2.0 mu M. The antioxidant capacity of OPC obtained by the Soxhlet extraction method from grape seeds was 2.85 U/mg. The recovery from the experiment in which OPC was added to grape seeds ranged from 97.0 to 98.6% (RSDs of 0.5-3.4%), suggesting a high accuracy in OPC detection. These findings are important because OPC is an internationally recognized antioxidant that eliminates free radicals in the human body and, therefore, may prevent a variety of diseases. Thus, we envisage that this Pt nanozyme-based assay may be prevalent for antioxidant capacity evaluation and analytical applications.
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