4.7 Article

SCHEEPDOG: Programming Electric Cues to Dynamically Herd Large-Scale Cell Migration

期刊

CELL SYSTEMS
卷 10, 期 6, 页码 506-+

出版社

CELL PRESS
DOI: 10.1016/j.cels.2020.05.009

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资金

  1. NCATS NIH HHS [TL1 TR003019] Funding Source: Medline
  2. NIGMS NIH HHS [R35 GM133574] Funding Source: Medline

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Directed cell migration is critical across biological processes spanning healing to cancer invasion, yet no existing tools allow real-time interactive guidance over such migration. We present a new bioreactor that harnesses electrotaxis-directed cell migration along electric field gradients-by integrating four independent electrodes under computer control to dynamically program electric field patterns, and hence steer cell migration. Using this platform, we programmed and characterized multiple precise, two-dimensional collective migration maneuvers in renal epithelia and primary skin keratinocyte ensembles. First, we demonstrated on-demand, 90-degree collective turning. Next, we developed a universal electrical stimulation scheme capable of programming arbitrary 2D migration maneuvers such as precise angular turns and migration in a complete circle. Our stimulation scheme proves that cells effectively time-average electric field cues, helping to elucidate the transduction timescales in electrotaxis. Together, this work represents an enabling platform for controlling cell migration with broad utility across many cell types.

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