期刊
IUCRJ
卷 7, 期 -, 页码 761-766出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2052252520007393
关键词
protein crystallography; X-ray topography; rocking curves; crystal perfection; oscillatory profiles; dynamical diffraction
资金
- PRESTO, Japan Science and Technology Agency [JPMJPR1995]
- Japan Society for the Promotion of Science (JSPS) KAKENHI [16K06708, 17K06797, 19K23579]
- Iketani Science and Technology Foundation [0291078-A]
- Grants-in-Aid for Scientific Research [19K23579, 16K06708] Funding Source: KAKEN
Knowledge of X-ray diffraction in macromolecular crystals is important for not only structural analysis of proteins but also diffraction physics. Dynamical diffraction provides evidence of perfect crystals. Until now, clear dynamical diffraction in protein crystals has only been observed in glucose isomerase crystals. We wondered whether there were other protein crystals with high quality that exhibit dynamical diffraction. Here we report the observation of dynamical diffraction in thin ferritin crystals by rocking-curve measurement and imaging techniques such as X-ray topography. It is generally known that in the case of thin crystals it is difficult to distinguish whether dynamical diffraction occurs from only rocking-curve profiles. Therefore, our results clarified that dynamical diffraction occurs in thin protein crystals because fringe contrasts similar to Pendellosung fringes were clearly observed in the X-ray topographic images. For macromolecular crystallography, it is hard to obtain large crystals because they are difficult to crystallize. For thin crystals, dynamical diffraction can be demonstrated by analysis of the equal-thickness fringes observed by X-ray topography.
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