期刊
FRONTIERS IN IMMUNOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2020.01117
关键词
monocytes; chemokine receptor; CX3CR1; CD43; multiparametric analysis
类别
资金
- Inserm
- Sorbonne University
- Fondation pour la recherche Medicale Equipe labelisee
- Agence Nationale de la Recherche, project CMOS (CX3CR1 expression on monocytes during sepsis) 2015 [ANR-EMMA-050]
- ANR
- FRM
In mice, monocytes (Mo) are conventionally described as CX3CR1(low)classical Mo (CMo) and CX3CR1(high)non-classical Mo (NCMo) based on the expression of EGFP in Cx3cr1(+/EGFP)mice and by analogy with human CX3CR1 expression. Although this terminology is widely used, it may not reflect the expression of CX3CR1 on Mo subsets. Using an unsupervised multiparametric analysis of blood Mo in steady state and after sterile peritonitis, we observed that CX3CR1 expression did not discriminate the CMo from the NCMo subsets. Our results highlight that despite being a reliable reporter to discriminate Mo subpopulations, EGFP level in Cx3cr1(+/EGFP)mice does not reflect CX3CR1 expression measured by a fluorescently-labeled CX3CL1 chemokine and a CX3CR1 specific antibody. In conclusion, authors should be cautious not to identify murine classical and non-classical Mo as CX3CR1(low)and CX3CR1(high)but rather use alternative markers such as the combination of Ly6C and CD43.
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