期刊
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
卷 76, 期 -, 页码 790-801出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2059798320009109
关键词
in situcrystallography; experimental phasing; crystal soaking; high throughput; isomorphism
资金
- Diamond Light Source from the UK Government
- Wellcome Trust [102593]
- Biotechnology and Biological Sciences Research Council
- Royal Society Wolfson Fellowship [RSWF\R2\182017]
- Wellcome Investigator Award in Science [210734/Z/18/Z]
- Wellcome Trust [210734/Z/18/Z] Funding Source: Wellcome Trust
In this article, a new approach to experimental phasing for macromolecular crystallography (MX) at synchrotrons is introduced and described for the first time. It makes use of automated robotics applied to a multi-crystal framework in which human intervention is reduced to a minimum. Hundreds of samples are automatically soaked in heavy-atom solutions, using a Labcyte Inc. Echo 550 Liquid Handler, in a highly controlled and optimized fashion in order to generate derivatized and isomorphous crystals. Partial data sets obtained on MX beamlines using anin situsetup for data collection are processed with the aim of producing good-quality anomalous signal leading to successful experimental phasing.
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