期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 7, 期 3, 页码 841-851出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01683
关键词
silk fibroin; nanofiber; biomineralization; gradient; tendon to bone
资金
- National Natural Science Foundation of China [11532004, 31600762, 11832008]
- Innovation and Attracting Talents Program for College and University (111 Project) [B06023]
- Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education [CQKLBST-2018-002]
The study developed a nanofibrous scaffold based on SF and SBF, successfully mimicking the structure and properties of natural interface tissue, promoting the growth and differentiation of bone marrow mesenchymal stem cells, and improving the ultimate load and fibrocartilage-like tissue formation at the tendon-to-bone interface.
Enthesis injury repair remains a huge challenge because of the unique biomolecular composition, microstructure, and mechanics in the interfacial region. Surgical reconstruction often creates new bone-scaffold interfaces with mismatched properties, resulting in poor osseointegration. To mimic the natural interface tissue structures and properties, we fabricated a nanofibrous scaffold with gradient mineral coating based on 10 x simulated body fluid (SBF) and silk fibroin (SF). We then characterized the physicochemical properties of the scaffold and evaluated its biological functions both in vitro and in vivo. The results showed that different areas of SF nanofibrous scaffold had varying levels of mineralization with disparate mechanical properties and had different effects on bone marrow mesenchymal stem cell growth and differentiation. Furthermore, the gradient scaffolds exhibited an enhancement of integration in the tendon-to-bone interface with a higher ultimate load and more fibrocartilage-like tissue formation. These findings demonstrate that the silk-based nanofibrous scaffold with gradient mineral coating can regulate the formation of interfacial tissue and has the potential to be applied in interface tissue engineering.
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