期刊
3 BIOTECH
卷 10, 期 8, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-020-02325-y
关键词
Authentication; Lyciumspecies; Molecular marker; Random amplified polymorphic DNA; Sequence-characterized amplified region
资金
- Science and Technology Innovation Team of Colleges and Universities in Sichuan Province [13TD0032]
- Joint Research Foundation of Luzhou City and Southwest Medical University [2018LZXNYD-YL01]
In the current study, ramp-PCR fragments from improved RAPD (random amplified polymorphic DNA) amplification ofLycium(Goji) species or cultivars were cut and cloned into the vector of pGEM-T. A positive clone 10-5 was screened by PCR amplification, enzymatic digestion, and Sanger sequencing. A SCAR (sequence-characterized amplified region) marker, named Goji 10-5, with 949 nucleotides in length, was identified. Goji 10-5 is specific toGojispeciesLycium chinenseMiller from Jiangxi in China and Texas in the USA. A BLAST search of this nucleotide sequence in the GenBank database indicated that it shows no identity with any other species, including no any otherLyciumspecies. As a new sequence, we have deposited it in the GenBank database with accession No. MN862323. PCR assays were developed and converted the nucleotide sequence to become a novel molecular marker forLycium chinenseMiller, named Goji 10-5. This marker may be used for the genetic identification of other samples. This study has successfully developed Goji 10-5, a specific SCAR marker to identifyL. chinenseand distinguish it from other species, including otherLyciumspecies from different locations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据