4.4 Article

Production of Chitosanase by Lentzea sp. OUR-I1 Using Acid-Pretreated Shrimp Shell in an Air-Lift Bioreactor and the Feasibility of Utilizing the Residual Biomass

期刊

WASTE AND BIOMASS VALORIZATION
卷 12, 期 5, 页码 2445-2458

出版社

SPRINGER
DOI: 10.1007/s12649-020-01191-2

关键词

Lentzea sp. OUR-I1; Air-lift bioreactor; Acid-treated SSP; Chitosanase; Chitin; Pigment

资金

  1. Coordinating Center for Thai Government Science and Technology Scholarship Students (CSTS) National Science and Technology Development Agency (NSTDA) [FDA-CO-2561-5753-TH]
  2. Thailand Research Fund [RTA6280014]

向作者/读者索取更多资源

Using abundant and inexpensive shrimp shell waste for pretreatment significantly increases chitosanase production, providing a cost-effective and environmentally friendly method for producing valuable bioproducts.
Chitosanase is an attractive enzymatic tool for the production of bioactive chitooligosaccharides. Nevertheless, its industrial use is restricted by its high cost and insufficient availability because chitosanase production needs an expensive inducer, chitosan. Therefore, this study developed a process to produce chitosanase from abundant and inexpensive shrimp shell waste entailing the pretreatment of shrimp shell powder (SSP) with 0.4 M acetic acid (AcSSP), which increased the substrate bioavailability and enhanced the chitosanase production by Lentzeasp. OUR-I1 four-fold. The initial flask-based process was scaled up into an air-lift bioreactor (ALB) and the maximum chitosanase activity (0.974 U/mL) was observed at an aeration rate of 2.0 vvm. This is the first report indicating the suitability of ALB for chitosanase production byLentzeasp. OUR-I1. Interestingly, Lentzeasp. OUR-I1 also produces a yellow pigment with prospective structure as carotenoids, indicating that the mycelium could be reused in the production of pigments. In addition, the fermented AcSPP displayed the FTIR spectrum of chitin and has high potential as an absorbent for methyl orange, methylene blue and coomassie brilliant blue, with a maximum decolorization efficiency of 73.21, 64.59 and 87.93%, respectively. The process developed represents a cost effective and environmentally friendly method of valorizing a waste product from the food industry into valuable bioproducts. [GRAPHICS] .

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据