期刊
STEM CELL REPORTS
卷 15, 期 1, 页码 171-184出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2020.05.005
关键词
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资金
- Bio & Medical Technology Development Program of NRF [2015M3A9C6028961, 2017M3A9F8072235, 2017M3A 9C6061284]
- Basic Science Research Program of NRF [2019 R1A2C2005681]
- MSICT of Republic of Korea
Human pluripotent stem cells (PSCs)through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryo-preserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine.
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