期刊
NANOMATERIALS
卷 10, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/nano10071309
关键词
nanoparticles; cerium dioxide; human placenta; cytotrophoblasts; syncytiotrophoblasts; cytotoxicity; pregnancy hormones
类别
资金
- ANSES, a French grant [BarBaPAhR N 2015-1-11329]
- Institut National de la Sante et de la Recherche Medicale (INSERM)
The human placenta is at the interface between maternal and fetal circulations, and is crucial for fetal development. The nanoparticles of cerium dioxide (CeO(2)NPs) from air pollution are an unevaluated risk during pregnancy. Assessing the consequences of placenta exposure to CeO(2)NPs could contribute to a better understanding of NPs' effect on the development and functions of the placenta and pregnancy outcome. We used primary villous cytotrophoblasts purified from term human placenta, with a wide range of CeO(2)NPs concentrations (0.1-101 mu g/cm(2)) and exposure time (24-72 h), to assess trophoblast uptake, toxicity and impact on trophoblast differentiation and endocrine function. We have shown the capacity of both cytotrophoblasts and syncytiotrophoblasts to internalize CeO(2)NPs. CeO(2)NPs affected trophoblast metabolic activity in a dose and time dependency, induced caspase activation and a LDH release in the absence of oxidative stress. CeO(2)NPs decreased the fusion capacity of cytotrophoblasts to form a syncytiotrophoblast and disturbed secretion of the pregnancy hormones hCG, hPL, PlGF, P4 and E2, in accordance with NPs concentration. This is the first study on the impact of CeO(2)NPs using human primary trophoblasts that decrypts their toxicity and impact on placental formation and functions.
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