期刊
FEBS OPEN BIO
卷 10, 期 10, 页码 1947-1956出版社
WILEY
DOI: 10.1002/2211-5463.12941
关键词
immunogenicity; monomer; peptide tag; protein solubility; SCP
资金
- JSPS [KAKENHI-15H04359, 18H02385]
- JSPS postdoctoral
- JSPS invitation fellowship (FY 2015)
- GARE (MOE, Bangladesh)
- TUAT's Institute of Global Innovation Research
- Japanese Government (Monbukagakusho: MEXT) PhD scholarship
- Grants-in-Aid for Scientific Research [18H02385] Funding Source: KAKEN
Poor immunogenicity of small proteins is a major hurdle in developing vaccines or producing antibodies for biopharmaceutical usage. Here, we systematically analyzed the effects of 10 solubility controlling peptide tags (SCP-tags) on the immunogenicity of a non-immunogenic model protein, bovine pancreatic trypsin inhibitor (BPTI-19A; 6 kDa). CD, fluorescence, DLS, SLS, and AUC measurements indicated that the SCP-tags did not change the secondary structure content nor the tertiary structures of the protein nor its monomeric state. ELISA results indicated that the 5-proline (C5P) and 5-arginine (C5R) tags unexpectedly increased the IgG level of BPTI-19A by 240- and 73-fold, respectively, suggesting that non-oligomerizing SCP-tags may provide a novel method for increasing the immunogenicity of a protein in a highly specific manner.
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