期刊
CARTILAGE
卷 13, 期 1_SUPPL, 页码 1494S-1510S出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1947603520938455
关键词
cartilage; osteoarthritis; histopathology; polarized light microscopy
类别
资金
- National Institute of Health [R01AR063733]
This study aimed to evaluate collagen orientation and arrangement in articular cartilage using polarized light microscopy, comparing it with standard histopathological methods. The results showed that PLM was effective in identifying early collagen alterations in the cartilage and could aid in improving the characterization of early primary OA cartilage.
Objective Evaluation of collagen orientation and arrangement in articular cartilage can improve our understanding of primary osteoarthritis (OA) progression and targeted therapies. Our goal was to determine if polarized light microscopy (PLM) for collagen organization is useful in identifying early primary OA features in comparison to current standard histopathological methods. Design Osteochondral specimens from 90 total knee arthroplasty patients with relatively preserved lateral femoral condyle were scored using (1) histological-histochemical grading system (HHGS); (2) Osteoarthritis Research Society International (OARSI); (3) PLM-Changoor system for repair cartilage, scores ranging between 0 (totally disorganized cartilage) and 5 (healthy adult cartilage); and (4) new PLM system for primary OA cartilage with superficial zone PLM (PLM-SZ) and deep zone PLM (PLM-DZ) scores, each ranging between 0 (healthy adult SZ and DZ collagen organization) and 4 (total loss of collagen organization). Serial sections were stained for collagen I and II antibodies. Spearman correlation coefficients (r(s)) were determined. Results The associations between: (1) PLM-Changoor and HHGS or OARSI were weak (r(s)= -0.36) or moderate (r(s)= -0.56); (2) PLM-SZ and HHGS or OARSI were moderate (r(s)= 0.46 orr(s)= 0.53); and (3) PLM-DZ and HHGS or OARSI were poor (r(s)= 0.31 orr(s)= 0.21), respectively. Specimens exhibiting early and mild OA (HHGS < 5 and OARSI < 8.6) had PLM-SZ and PLM-DZ scores between 0 and 4 and between 0 and 3, respectively, and indicated new histopathological features not currently considered by HHGS/OARSI. Conclusions PLM was effective at identifying early SZ and DZ collagen alterations that were not evident in the traditional scoring systems. Incorporating PLM scores and/or additional HHGS/OARSI features can help improve characterization of early primary OA cartilage.
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