Stimuli-responsive poly(N-isopropyl acrylamide)-co-tyrosine@gadolinium: Iron oxide nanoparticle-based nanotheranostic for cancer diagnosis and treatment

In this paper, we have prepared a stimuli-responsive polymer modified gadolinium doped iron oxide nanoparticle (poly@Gd-MNPs) as cancer theranostic agent. The responsive polymer is composed of the poly(N-isopropyl acrylamide)-co-tyrosine unit, which shows excellent loading for the anti-cancer drug (methotrexate) and stimuli dependent release (change in pH and temperature). The in vitro experiment revealed that the poly@Gd-MNPs exhibited T-1-weighted MRI capability (r(1) = 11.314 mM(-1) s(-1)) with good in-vitro hyperthermia response. The prepared poly@Gd-MNPs has generated quick heating (45 degrees C in 2 min) upon exposure to an alternating magnetic field and able to travel a distance of 35 cm in 1 min in the presence of an external magnet. The poly@Gd-MNPs shows 86% of drug loading capacity with 70% drug release in first 2 h. The cytotoxic assay (MTF) demonstrated that the nanoparticle did not affect the viability of normal human fibroblast and efficiently kill the MCF7 cancer cells in the presence of an external magnetic field. To explore the uptake of poly@Gd-MNPs in the cells, bright field cell imaging study was also performed. This study provides a valuable approach for the design of highly sensitive polymer modified gadolinium doped iron oxide-based T-1 contrast agents for cancer theranostics. (C) 2016 Elsevier B.V. All rights reserved.