期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 146, 期 -, 页码 468-474出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.06.043
关键词
Cobalt ferrite; Structure modification; Bovine hemoglobin absorption; Protein separation
资金
- National Natural Science Foundation of China [21574009, 51521062]
- Beijing Natural Science Foundation [2142026]
- Beijing collaborative innovative research center for cardiovascular diseases
- Innovation and Promotion Project of Beijing University of Chemical Technology
Ultrafine cobalt ferrite (CoFe2O4) nanoparticles with narrow size distributions and regular morphologies were prepared through an improved procedure based on the high-temperature diol reduction method. By replacing the original reducing agent like expensive dihydric alcohol, oleylamine was applied as both stabilizer and reducing agent, which benefit for large scale synthesis of magnetic CoFe2O4 nanoparticles. To produce protein-separation agent with both high specific surface area and magnet content, inverse microemulsion system and substitution reaction were employed to render the produced CoFe2O4 nanoparticles with dense silica bilayer and iminodiacetic acid (IDA)-like structure modification. After the chelation with Cu2+, the metal-immobilized CoFe2O4 nanoparticles can specifically absorb bovine hemoglobin (BHb) with maximum adsorption capacity as q(m) = 1812.3 mg/g. Compared with the reported Cu2+-immobilized magnetic nanoparticles, the as-prepared CoFe2O4@Si-IDA-Cu2+ nanoparticles exhibited excellent adsorption capacity and were applied as high efficient protein separation agent in a real complex biological fluid like bovine blood. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据