期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 145, 期 -, 页码 64-71出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.04.042
关键词
Melatonin; Nano structured lipid carrier (NLC); Apoptosis; MCF-7; Tamoxifen
资金
- Drug applied research center, Tabriz University of Medical Sciences, Tabriz, Iran
Background: Finding advanced anti-cancer agents with selective toxicity in tumor tissues is the goal of anticancer delivery systems. This study investigated potential application of nanostructured lipid carriers (NLCs) in increasing melatonin induced cytotoxicity and apoptosis in MCF-7 breast cancer cells. Methods: Melatonin-loaded NLCs were characterized for particle size, zeta potential, Fourier transforms infrared spectroscopy, differential scanning calorimetry, cellular uptake, and scanning electron microscope (SEM). Anti-proliferative and apoptotic effects of new formulation were evaluated by MTT and flow cytometric assays, respectively. Gene expression of apoptotic markers including survivin, Bcl-2 and Bid were examined by Real time quantitative PCR. Results: The optimized formulation of NLCs revealed mean particle size of 71 +/- 5 nm with nearly narrow size distribution. The formulation exhibited an acceptable stability during four months in terms of size and lack of drug release. The IC50 values for melatonin and tamoxifen were 1.3 +/- 0.4 mM and 30.7 +/- 5.2 mu M, respectively. Melatonin loaded NLCs decreased percentage of cell proliferation from 55 +/- 7.2% to 40 +/- 4.1% (p < 0.05). Co-treatment of the cells with melatonin loaded nanoparticles and tamoxifen caused two fold increase in the percentage of apoptosis (p < 0.05). Evaluation of gene expression profile demonstrated a marked decrease in anti-apoptotic survivin with increase in pro-apoptotic Bid mRNA levels. Conclusion: Taken together, our results suggest NLC technology as a promising delivery system, which elevates the efficacy of chemotherapeutics in breast cancer cells. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据