期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 145, 期 -, 页码 95-103出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.04.027
关键词
DOX; Oleic acid; NLCs; SAXS; pH-dependent drug release
资金
- Shanghai Natural Science Foundation [15ZR1409900]
- National Natural Science Foundation of China [21573070]
- Fundamental Research Funds for the Central Universities
- Knowledge Innovation Program of CAS [2013KIP103]
The hydrophilic drug Doxorubicin hydrochloride (DOX) paired with oleic acid (OA) was successfully incorporated into nanostructured lipid carriers (NLCs) by a high-pressure homogenization (HPH) method. Drug nanovehicles with proper physico-chemical characteristics (less than 200nm with narrow size distribution, spherical shape, layered internal organization, and negative electrical charge) were prepared and characterized by dynamic light scattering, zeta potential measurements, transmission electron microscopy, small-angle X-ray scattering and differential scanning calorimetry. The drug loading and entrapment efficiency of DOX-OA/NLCs were 4.09% and 97.80%, respectively. A pH-dependent DOX release from DOX-OA/NLCs, i.e., fast at pH 3.8 and 5.7 and sustained at pH 7.4, was obtained. A cytotoxicity assay showed that DOX-OA/NLCs had comparable cytotoxicity to pure DOX and were favorably taken up by HCT 116 cells. The intracellular distribution of DOX was also studied using a confocal laser scanning microscope. All of these results demonstrated that DOX-OA/NLCs could be a promising drug delivery system with tumor-specific DOX release for cancer treatment. (C) 2016 Elsevier B.V. All rights reserved.
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