期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 146, 期 -, 页码 1-8出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.05.039
关键词
Mineralization; Calcium carbonate; Nitric oxide; Nanocarrier; Intracellular delivery
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2012R1A5A2051388]
- Korea Health Technology R&D project through the KHIDI - Ministry of Health & Welfare, Republic of Korea [HI14C0175]
- National Research Foundation of Korea [2012R1A5A2048310, 2012R1A5A2051388] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We describe a calcium carbonate (CaCO3) mineralization approach to generate pH-responsive nanocarriers that can stably load S-nitrosoglutathione (GSNO) and dissolve at acidic endosomes to trigger intracellular release of nitric oxide (NO). GSNO-loaded CaCO3-mineralized nanoparticles (GSNO-MNPs) were prepared by an anionic block copolymer (PEG-Poly(L-aspartic acid))-templated mineralization. Ionic GSNO could be loaded in situ inside the CaCO3 core during the mineralization process. The stability of GSNO shielded within the crystalline CaCO3 core was greatly enhanced. The GSNO-MNPs triggered NO release at endosomal pH and an intracellular ascorbic acid level. Confocal microscopy demonstrated that the GSNO-MNPs could be dissolved at endosomal environments to release GSNO and sequentially generate NO through the GSNO reduction in the cytosol. In vitro cell experiments demonstrated that NO release by the GSNO-MNPs efficiently improved therapeutic activity of doxorubicin (DOX). (C) 2016 Elsevier B.V. All rights reserved.
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