期刊
MICROMACHINES
卷 11, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/mi11060577
关键词
dose-response; droplet-based; segmented-flow; photo-fluorimetric; flow sensor; dynamic cultivation; screening; bacterial; microbial; proliferation; droplet generator
类别
资金
- Thuringer Aufbaubank, Project Screen In Drop Line [2016FE9016]
Droplet-based microfluidics is a versatile tool to reveal the dose-response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose-response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose-response data for 1D and 2D dose-response screenings are presented.
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