期刊
MICROMACHINES
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/mi11080767
关键词
electroporation; membrane resealing; electrotransfection; live cell staining; cell adhesion
类别
资金
- Research Grants Council of the Hong Kong Special Administration Region [GRF/17257016, GRF/17210618]
- National Natural Science Foundation of China [11872325]
A novel electroporation system was developed to introduce transient membrane pores to cells in a spatially and temporally controlled manner, allowing us to achieve fast electrotransfection and live cell staining as well as to systematically interrogate the dynamics of the cell membrane. Specifically, using this platform, we showed that both reversible and irreversible electroporation could be induced in the cell population, with nano-sized membrane pores in the former case being able to self-reseal in similar to 10 min. In addition, green fluorescent protein(GFP)-vinculin plasmid and 543 phalloidin have been delivered successively into fibroblast cells, which enables us to monitor the distinct roles of vinculin and F-actin in cell adhesion and migration as well as their possible interplay during these processes. Compared to conventional bulk electroporation and staining methods, the new system offers advantages such as low-voltage operation, cellular level manipulation and testing, fast and adjustable transfection/staining and real-time monitoring; the new system therefore could be useful in different biophysical studies in the future.
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