期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 146, 期 -, 页码 204-211出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.05.094
关键词
Hydrogel; Pluronic F127; Carboxymethyl hexanoyl chitosan; Cell encapsulation
资金
- Ministry of Science and Technology, Taiwan [103-2622-E-011-024-CC2]
This study demonstrated a novel hydrogel system composing of Pluronic F127, carboxymethyl hexanoyl chitosan (CA) and glutaraldehyde (GA) for encapsulating fibroblasts (L-929). The thermal behavior of the hydrogel was evaluated using TGA, the swelling behavior of the hydrogel was evaluated in Dulbecco's Modified Eagle's medium (DMEM), and the mechanical properties were determined through dynamic mechanical analysis. Cells were encapsulated by simple mixing, and the viability of encapsulated cells was determined using alamar blue cell viability assay and the cells morphology was examined using fluorescent imaging. The results indicated that the T-gel of this system was around 30 degrees C, where sol-gel transformation occurred within 90s. Although the addition of CA and GA reduced the shear moduli slightly, the F127/CA/GA gel was able to remain in gelling state in the medium for more than 1 month. In vitro cell culture study revealed that F-127/CA/GA hydrogels were non-cytotoxic. Moreover, the viability of encapsulated L929 was 106% after incubation for 5 days. Based on these results, these F127/CA/GA hydrogels can be used to encapsulate cells for tissue engineering applications. (C) 2016 Elsevier B.V. All rights reserved.
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