期刊
MEDICAL SCIENCE MONITOR
卷 26, 期 -, 页码 -出版社
INT SCIENTIFIC INFORMATION, INC
DOI: 10.12659/MSM.925545
关键词
Medicine, Chinese Traditional; Molecular Mechanisms of Pharmacological Action; Osteoarthritis; Computer Communication Networks; Achyranthes
资金
- National Natural Science Foundation of China [81774345]
- Natural Science Foundation of Fujian Province [2018J01874]
- Developmental Fund of Chen Keji Integrative Medicine [CKJ2020005]
- Science and Technology project of Traditional Chinese Medicine of Fujian Province [2017FJZYJC204]
Backgrounds: Achyranthes bidentata is a Chinese traditional herbal medicine widely used to treat osteoarthritis (OA). This study aimed to identify active compounds from Achyranthes bidentata through systematic pharmacology and in vitro experiments to find the targets of Achyranthes bidentata in the treatment of OA. Material/Methods: We screened the active compounds of Achyranthes bidentata from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Then, we used STITCH and Open Targets Platform databases to screen the active components and predict the potential targets of Achyranthes bidentata in the treatment of OA. Subsequently, we studied the compound-target network and protein interaction network and analyzed the enrichment of potential target proteins. Finally, we used Western blot analysis to verify the therapeutic effect of Achyranthes bidentata extract on the expression of OA-related target proteins. Results: There were 7 active components in Achyranthes bidentata, which were strongly related to the 74 targets of OA. Quercetin, baicalein, and berberine are the critical active compounds of Achyranthes bidentata in the treatment of OA. Protein interaction analysis and in vitro experiments suggested that TNIF,11-6, and TP53 are the critical targets of Achyranthes bidentata in the treatment of OA. Functional enrichment analysis showed that Achyranthes bidentata plays a pharmacological role in OA through apoptosis, inflammation, and immune regulation. Conclusions: Quercetin, baicalein, and berberine are the critical active compounds of Achyranthes bidentata in the treatment of OA. TNF, IL-6, and TP53 may be potential targets for the treatment of OA.
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