期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 147, 期 -, 页码 217-223出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.08.006
关键词
Chitosan; Chondroitin sulfate; Nano-hydroxyapatite; In situ
资金
- National Natural Science Foundation of China [31100677, 31370958]
- Scientific Project of Fujian Education Department [JA14031]
- Guangdong Natural Science Funds for Distinguished Young Scholar [2016A030306018]
The fabrication of bone scaffolds with interconnected porous structure, adequate mechanical properties and excellent biocompatibility presents a great challenge. Herein, a hybrid nanostructured chitosan/chondroitin sulfate/hydroxyapatite (ChS/CSA/HAP) in situ composite scaffold was prepared by in situ fabrication and freeze-drying technique. The composition and morphology of scaffold were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM). It proved that the low crystallinity of HAP crystals were uniformly distributed in ChS/CSA organic matrix and the nanostructured hybrid scaffold exhibited good mechanical property. The biocompatibility and in vitro bioactivity were detected by MTT-assay, maturation (alkaline phosphatase (ALP) activity), Hoechst 33258 and PI fluorescence staining. In vitro tests indicated that the hybrid scaffold not only promoted the adhesion and proliferation of osteoblasts, but also improved the growth of the osteoblasts. Therefore, it is promising for bone repair application in bone tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据