WRKY Transcription Factor OsWRKY29 Represses Seed Dormancy in Rice by Weakening Abscisic Acid Response

For efficient plant reproduction, seed dormancy delays seed germination until the environment is suitable for the next generation growth and development. The phytohormone abscisic acid (ABA) plays important role in the induction and maintenance of seed dormancy. Previous studies have identified that WRKY transcription factors can regulate ABA signaling pathway. Here, we identified anOswrky29mutant with enhanced dormancy in a screen of T-DNA insertion population. OsWRKY29 is a member of WRKY transcription factor family which located in the nuclear. The genetic analyses showed that both knockout and RNAi lines ofOsWRKY29had enhanced seed dormancy whereas its overexpression lines displayed reduced seed dormancy. When treated with ABA,OsWRKY29knockout and RNAi lines showed greater sensitivity than its overexpression lines. In addition, the expression levels of ABA positive response factorsOsVP1andOsABF1were higher in theOsWRKY29mutants but were lower in its overexpression lines. Further assays showed that OsWRKY29 could bind to the promoters ofOsABF1andOsVP1to inhibit their expression. In summary, we identified a new ABA signaling repressor OsWRKY29 that represses seed dormancy by directly downregulating the expression ofOsABF1andOsVP1.