期刊
FRONTIERS IN PLANT SCIENCE
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2020.00749
关键词
pentatricopeptide repeat (PPR) proteins; RNA editing; mitochondria; chloroplasts; sterility; OsMORFs
资金
- National Key Research and Development Program of China [2016YFD0100804]
- National Natural Science Foundation of China [31371698, 31670310, 31871592]
- Suzhou Science and Technology Project [SNG2017061]
- State Key Laboratory for Conservation and Utilization of Subtropical AgroBioresources [SKLCUSA-b201717]
- Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University [PTJH12004]
In land plants, the pentatricopeptide repeat (PPR) proteins form a large family involved in post-transcriptional processing of RNA in mitochondria and chloroplasts, which is critical for plant development and evolutionary adaption. Although studies showed a number of PPR proteins generally influence the editing of organellar genes, few of them were characterized in detail in rice. Here, we report a PLS-E subclass PPR protein in rice, PPR756, loss of function of which led to the abolishment of RNA editing events among three mitochondrial genes includingatp6,ccmC, andnad7. Their defective C-to-U transformation then resulted in improper amino acid retention which could cause abortive pollen development. Furthermore, PPR756 could bind to the three target genes directly and interact with three OsMORFs (multiple organellar RNA editing factors): OsMORF1, OsMORF8-1, and OsMORF8-2. The knock-out plants ofPPR756exhibited retarded growth and greener leaves during the early vegetative stages, along with sterile pollen and lower seed setting at the reproductive stage. These results established a role for PPR756 in rice development, participating in RNA editing of three various transcripts and cooperating with OsMORFs via an editosome manner in rice.
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