期刊
FRONTIERS IN MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.01331
关键词
sulfite resistance; domestication; translocation; multiplex PCR; marker assisted selection
类别
资金
- Biolaffort Company
- Region Aquitaine Grant SESAM
- Fondo para la Investigacion Cientifica y Tecnologica (FONCyT) (Argentina) [PICT-20143113]
- SUV2015 (Universidad Catolica de Cordoba)
- National Research Council of Argentina (CONICET)
Chromosomal rearrangements (CR) such as translocations, duplications and inversions play a decisive role in the adaptation of microorganisms to specific environments. In enologicalSaccharomyces cerevisiaestrains, CR involving the promoter region of the geneSSU1lead to a higher sulfite tolerance by enhancing the SO(2)efflux. To date, three differentSSU1associated CR events have been described, including translocations XV-t-XVI and VIII-t-XVI and inversion inv-XVI. In the present study, we developed a multiplex PCR method (SSU1checkup) that allows a rapid characterization of these three chromosomal configurations in a single experiment. Nearly 600S. cerevisiaestrains collected from fermented grape juice were genotyped by microsatellite markers. We demonstrated that alleles of theSSU1promoter are differently distributed according to the wine environment (cellar versus vineyard) and the nature of the grape juice. Moreover, rearrangedSSU1promoters are significantly enriched among commercial starters. In addition, the analysis of nearly isogenic strains collected in wine related environments demonstrated that the inheritance of these CR shapes the genetic diversity of clonal populations. Finally, the link between the nature ofSSU1promoter and the tolerance to sulfite was statistically validated in natural grape juice containing various SO(2)concentrations. TheSSU1checkup is therefore a convenient new tool for addressing population genetics questions and for selecting yeast strains by using molecular markers.
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