期刊
FRONTIERS IN MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.01583
关键词
coronavirus; genome; RNA; G-quadruplex; inverted repeats
类别
资金
- Ministry of Education, Youth and Sports of the Czech Republic in the National Feasibility Program I [LO1208 TEWEP]
- EU structural funding Operational Programme Research and Development for innovation [CZ.1.05/2.1.00/19.0388]
- University of Ostrava [SGS/01/PrF/2020, SGS/07/PrF/2020]
Non-canonical nucleic acid structures play important roles in the regulation of molecular processes. Considering the importance of the ongoing coronavirus crisis, we decided to evaluate genomes of all coronaviruses sequenced to date (stated more broadly, the orderNidovirales) to determine if they contain non-canonical nucleic acid structures. We discovered much evidence of putative G-quadruplex sites and even much more of inverted repeats (IRs) loci, which in fact are ubiquitous along the whole genomic sequence and indicate a possible mechanism for genomic RNA packaging. The most notable enrichment of IRs was found inside 5 ' UTR for IRs of size 12+ nucleotides, and the most notable enrichment of putative quadruplex sites (PQSs) was located before 3 ' UTR, inside 5 ' UTR, and before mRNA. This indicates crucial regulatory roles for both IRs and PQSs. Moreover, we found multiple G-quadruplex binding motifs in human proteins having potential for binding of SARS-CoV-2 RNA. Non-canonical nucleic acids structures inNidoviralesand in novel SARS-CoV-2 are therefore promising druggable structures that can be targeted and utilized in the future.
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