Leptomonas seymouriCo-infection in Cutaneous Leishmaniasis Cases Caused byLeishmania donovaniFrom Himachal Pradesh, India

Himachal Pradesh in India is a newer endemic state with co-existence of cutaneous and visceral leishmaniasis. The cutaneous leishmaniasis cases are on an increase in the region and reported to be unusually caused byLeishmania donovaniwith limited molecular validation. In order to molecularly characterize the causative parasite of the cutaneous disease, parasite specific Internal-Transcribed Spacer 1 (ITS1) PCR RFLP and sequence analysis was performed on skin lesional biopsies from cutaneous leishmaniasis patients. Interestingly, we found the presence ofLeptomonas seymouriin 38.5% (22/57) of the patients along withL. donovanidetected in all the samples.L. seymouriis a monoxenous insect trypanosomatid, generally incapable of infecting humans. In recent years, the parasite is also reported to co-infect humans withL. donovaniin visceral and post kala-azar dermal leishmaniasis (PKDL) cases prevalent in northeastern India. The finding ofL. seymouri-L. donovanico-infection in unusual cutaneous cases from Himachal Pradesh is the first ever to our knowledge and imply a newer disease paradigm. There is an urgent need to understand the biology ofLeptomonasco-infection withL. donovaniand its possible role in visceral and/or dermotropic disease outcome. Importantly,L. seymourico-infection in cutaneous cases and previously reported visceral and PKDL cases needs to be recognized as a newer phenomenon by the leishmaniasis surveillance program in India.