The genus Madurella: Molecular identification and epidemiology in Sudan

Eumycetoma (mycotic mycetoma) is the fungal form of mycetoma, a subcutaneous infection occurring in individuals living in endemic areas of the disease. The Sudan is hyperendemic for mycetoma, with the highest incidence being reported from Gezira State, Central Sudan. The present study was conducted at the Gezira Mycetoma Center and aimed to determine the cause of black-grain eumycetoma in the state and describe its epidemiology. Black-grain specimens were collected during the surgical operation and direct detection of the causative agent was performed usingM.mycetomatisspecies-specific PCR and ITS PCR followed by sequencing. Black-grain was reported from 93.3% of all confirmed mycetoma cases (n = 111/119), with a prevalence in young males. Of the 91 samples subjected to direct PCR, 90.1% (n = 82) gave positive results. The predominant species (88.2%) wasMadurella mycetomatis. One sample was identified asM.fahalii, one asM.tropicana, and one matched the phytopathogenic speciesSphaerulina rhododendricola. The highest endemic zones were Southern Gezira (76.6%) and Northern Sinnar (23.4%). The study confirmed that direct molecular detection on grains provides rapid and specific diagnosis of agents of eumycetoma. Author summary Eumycetoma is a neglected fungal disease endemic in Africa, India, and Latin America. Black-grain eumycetoma is the most common type in Africa and is mainly caused byMadurellaspp. The Sudan, and in particular Gezira State, central Sudan is hyperendemic for black-grain eumycetoma. Patients with this type of mycetoma are treated with surgery in combination with antifungal therapy. In this study, we collected surgical biopsies from patients attending Gezira Mycetoma Center to directly identify the etiology of black-grain eumycetoma in this state. We also studied the epidemiology of the disease based on the demography of the patients' population. Our result showed that the highest endemic regions were Southern Gezira (76.6%) and Northern Sinnar (23.4%). By applying direct PCR and sequencing we confirmed that the most common etiology of the disease isMadurella mycetomatis(88.2%). In addition, we found one case ofM.fahaliiand the first Sudanese case ofM.tropicanaandSphaerulina rhododendricola.