期刊
JOURNAL OF INTEGRATIVE AGRICULTURE
卷 19, 期 7, 页码 1721-1730出版社
ELSEVIER SCI LTD
DOI: 10.1016/S2095-3119(20)63224-5
关键词
reduced height gene; wheat; QTL; BSA; molecular marker
资金
- National Key Research and Development Program of China [2016YFD0102100, 2016YFD0101802]
- National Natural Science Foundation of China [31801346]
- earmarked fund for China Agriculture Research System [CARS-03]
Low stature in wheat is closely associated with lodging resistance, and this impacts harvest index and grain yield. The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding. In this study, we identified an EMS-induced dwarf wheat mutant JE0124 from the elite cultivar Jing411. JE0124 possesses increased stem strength and a 33% reduction in plant height compared with wild type. Gibberellic acid (GA) treatment analysis suggested that JE0124 was GA-sensitive. Analysis of the frequency distribution of plant height in four F-2 populations derived from crosses between JE0124 and the relatively taller varieties Nongda 5181 and WT indicated that the dwarfism phenotype was quantitatively inherited. We used two F-2 populations and 312 individuals from the reciprocal cross of Nongda 5181 and JE0124 to map the quantitative trait locus (QTL) for reduced height to a 0.85-cM interval on chromosome 2DL. The mapping was done by using a combination of 660K SNP array-based bulked segregant analysis (BSA) and genetic linkage analysis, with logarithm of odds (LOD) scores of 5.34 and 5.78, respectively. Additionally, this QTL accounted for 8.27-8.52% of the variation in the phenotype. The dwarf mutant JE0124 and the newly discovered dwarfing gene on chromosome 2DL in this study will enrich genetic resources for dwarf wheat breathing.
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