期刊
CELL REPORTS
卷 32, 期 3, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2020.107942
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资金
- Centre National de la Recherche Scientifique (CNRS)
- Fondation pour la Recherche Medicale (FRM, programme Equipes 2019)
- l'Agence National pour la Recherche [ANR-16-CE12-0022-01]
- LabEx Who Am I?'' [ANR-11-LABX-0071]
- Universite de Paris IdEx - French Government through its Investments for the Future'' program [ANR-18IDEX-0001]
- Agence Nationale de la Recherche (ANR) [ANR-16-CE12-0022] Funding Source: Agence Nationale de la Recherche (ANR)
A large share of the non-coding transcriptome in yeast is controlled by the Nrd1-Nab3-Sen1 (NNS) complex, which promotes transcription termination of non-coding RNA (ncRNA) genes, and by the nuclear exosome, which limits the steady-state levels of the transcripts produced. How unconstrained ncRNA levels affect RNA metabolism and gene expression are long-standing and important questions. Here, we show that degradation of ncRNAs by the exosome is required for freeing Nrd1 and Nab3 from the released transcript after termination. In exosome mutants, these factors are sequestered by ncRNAs and cannot be efficiently recycled to sites of transcription, inducing termination defects at NNS targets. ncRNA-dependent, genome-wide termination defects can be recapitulated by the expression of a degradation-resistant, circular RNA containing a natural NNS target in exosome-proficient cells. Our results have important implications for the mechanism of termination, the general impact of ncRNAs abundance, and the importance of nuclear ncRNA degradation.
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