期刊
ACS SYNTHETIC BIOLOGY
卷 9, 期 7, 页码 1823-1832出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.0c00172
关键词
multigene expression; Type III PKSs; coumaroyl-CoA ligase; chalcone synthase; naringenin
资金
- Leibniz Research Cluster (LRC)
- European Social Fund ESF Europe for Thuringia project SphinX
Combinatorial biosynthesis has great potential for designing synthetic circuits and amplifying the production of new active compounds. Studies on multienzyme cascades are extremely useful for improving our knowledge on enzymatic catalysis. In particular, the elucidation of enzyme substrate promiscuity can be potentially used for bioretrosynthetic approaches, leading to the design of alternative and more convenient routes to produce relevant molecules. In this perspective, plant-derived polyketides are extremely adaptable to those synthetic biological applications. Here, we present a combination of an in vitro CoA ligase activity assay coupled with a bacterial multigene expression system that leads to precursor-directed biosynthesis of 21 flavonoid derivatives. When the vast knowledge from protein databases is exploited, the herein presented procedure can be easily repeated with additional plant-derived polyketides. Lastly, we report an efficient in vivo expression system that can be further exploited to heterologously express pathways not necessarily related to plant polyketide synthases.
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