期刊
ACS SYNTHETIC BIOLOGY
卷 9, 期 7, 页码 1771-1780出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.0c00137
关键词
DNA replication; end replication problem; repetitive sequence replication; isothermal replication; phi29 DNA polymerase
资金
- Japan Society for the Promotion of Science, KAKENHI [JP15KT0080, JP15H04407, JP17J01023]
- Innovation inspired by Nature Research Support Program, Sekisui Chemical Co., Ltd.
- Astrobiology Center Program of National Institutes of Natural Sciences (NINS) [AB021005]
DNA replication is one of the central functions of the cell. The complexity of modern DNA replication systems raises a question: is it possible to achieve a simpler continuous isothermal DNA replication using fewer proteins? Here, we searched such replication using an evolutionary approach. Through a long-term serial dilution experiment with phi29 DNA polymerase, we found that large repetitive DNAs spontaneously appear and continuously replicate. The repetitive sequence is critical for replication. Arbitrary sequences can replicate if they contain many repeats. We also demonstrated continuous DNA replication using expressed polymerase from the DNA for 10 rounds. This study revealed that continuous isothermal DNA replication can be achieved in a scheme simpler than that employed by modern organisms, providing an alternative strategy for simpler artificial cell synthesis and a clue to possible primitive forms of DNA replication.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据