期刊
STEM CELL RESEARCH & THERAPY
卷 11, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13287-020-01735-2
关键词
Liver fibrosis; Mesenchymal stem cell; Schistosomiasis; Hepatic granulomas; TLR; Immune modulation
资金
- Double First-Class University Project grant from the South China University of Technology of China
- Open Project of Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education [2019kfkt03]
Background and aimsA marked egg-induced CD4(+) T cell programmed inflammation and subsequent hepatic fibrosis characterize the pathogenesis of schistosomiasis. Mesenchymal stem cell (MSC) has been extensively studied for the treatment of schistosomiasis. However, the mechanism by which MSCs modulate the pathogenesis of schistosomiasis has not been clarified. Furthermore, the local inflammatory milieu may greatly influence the immunoregulatory properties of MSCs, and our early experiments demonstrated that Toll-like receptor (TLR)2/TLR4 agonist effected immune modulation of MSC. Here, we further investigated their modulation on the pathogenesis of schistosomiasis.MethodsAdult BALB/c male mice were percutaneously infected with 162 pairs S. japonicum cercariae and received intravenously pretreated MSC at 1week and 3weeks post-infection, respectively. At 8weeks post-infection, effects of MSC on liver histology were shown by hematoxylin and eosin (H&E) staining and Masson staining and quantitatively compared by the hepatic hydroxyproline content; alpha-smooth muscle actin (alpha-SMA), collagen type I(Col-1), transforming growth factor beta (TGF-beta), and tumor necrosis factor-alpha (TNF-alpha) gene expression in the liver were assessed by semi-quantitative polymerase chain reaction (PCR); the Th1/Th2 dominance among different groups was compared by analyzing CD4(+) interferon-gamma (IFN-gamma)+ and CD4+interleukin-4 (IL-4)+T cells in the liver by flow cytometry and serum level of IFN-gamma and IL-5 using enzyme-linked immunosorbent assay (ELISA). Effects of different kinds of MSC were further evaluated in vitro by the coculture system.ResultsResults showed TLR4- and IFN-gamma-activated MSC alleviated liver fibrosis in infected mice, without a significant increase of mortality, and unpretreated MSC showed no clear improvement; however, TLR2- and IFN-gamma-activated MSC displayed aggravated immunopathology. In accord with the pathological results, TLR4- and IFN-gamma-activated MSC groups showed moderate enhancement of Th1 response in vitro and clear Th1 dominance in vivo without leading to extreme inflammation, whereas TLR2- and IFN-gamma-activated MSC not only induced Th1 response, but also triggered excessive inflammation as evidenced by atrophy of the thymus and higher TNF level in the coculture system.Conclusions This study demonstrates that TLR4 combined with IFN-gamma can activate the MSC group with positive effects on the pathology of schistosomiasis by modulating Th subsets at some degree. This result suggests that when MSC is being used to treat different immuno-disturbance complications, subtle pretreatment methods should be seriously considered.
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