期刊
NUTRIENTS
卷 12, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/nu12082421
关键词
LEAAs; resistance exercise; mTORC1; ubiquitin; inflammation
资金
- Ajinomoto Co., Inc.
Resistance exercise transiently activates anabolic and catabolic systems in skeletal muscle. Leucine-enriched essential amino acids (LEAAs) are reported to stimulate the muscle anabolic response at a lower dose than whey protein. However, little is known regarding the effect of LEAA supplementation on the resistance exercise-induced responses of the anabolic and catabolic systems. Here, we conducted a randomized, double-blind, placebo-controlled, parallel-group comparison trial to investigate the effect of LEAA supplementation on mechanistic target of rapamycin complex 1 (mTORC1), the ubiquitin-proteasome system and inflammatory cytokines after a single bout of resistance exercise in young men. A total of 20 healthy young male subjects were supplemented with either 5 g of LEAA or placebo, and then they performed 10 reps in three sets of leg extensions and leg curls (70% one-repetition maximum). LEAA supplementation augmented the phosphorylation of mTOR(Ser2448)(+77.1%,p< 0.05), p70S6K(Thr389)(+1067.4%,p< 0.05), rpS6(Ser240/244)(+171.3%,p< 0.05) and 4EBP1(Thr37/46)(+33.4%,p< 0.05) after resistance exercise. However, LEAA supplementation did not change the response of the ubiquitinated proteins, MuRF-1 and Atrogin-1 expression. Additionally, the mRNA expression of IL-1 beta and IL-6 did not change. These data indicated that LEAA supplementation augments the effect of resistance exercise by enhancing mTORC1 signal activation after exercise.
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