Dendropanax MorbiferaExtract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line

Dendropanax morbiferais a versatile plant that has been used as a herbal medicine due to its various useful medicinal effects. To protect its active component from biological stress and increase its drug efficacy as well as drug bioavailability, nanoemulsion was prepared.Dendropanax morbiferazinc oxide nanoparticles (DM-ZnO NPs) were synthesized using the plant extract via the co-precipitation method and loaded with active indole-3-carbinol for nanoemulsion formulation using the ultrasonication process. Field emission transmission electron microscope revealed the flower shape of theDendropanax morbiferaindole-3-carbinol zinc oxide nanoemulsion (DM-ZnO-I3C-NE). In contrast,DM-ZnO NPs showed a spheroid shape that coincides agreeably with field emission electron scanning microscope. The hydrodynamic sizes by dynamic light scattering are about 65 +/- 3 nm and 239.6 +/- 6 nm and the crystallite sizes from X-ray diffraction are 11.52 nm and 16.07 nm forDM-ZnO NPs andDM-ZnO-I3C-NE, respectively. In vitro analysis revealed the cytotoxicity ofDM-ZnO-I3C-NE against a human lung cancer cell line (A549) at 12.5 mu g/mL as well as reactive oxygen species (ROS) production. TheDM-ZnO-I3C-NE-induced ROS generation level was higher than that ofDM-ZnO NPs and free indole-3-carbinol. The synergistic effect ofDM-ZnO and indole-3-carbinol indicatesDM-ZnO-I3C-NE as a potential candidate for future lung cancer drug and could be scope for functional food.