In Vitro Studies of Ce-Doped Hydroxyapatite Synthesized by Sol-Gel Method for Biomedical Applications

Purpose The present study was focused on the synthesis and characterization of cerium with hydroxyapatite for biomedical applications. Method Hydroxyapatite (HAp) substituted with variable concentration (0.075, 0.150, 0.225%) of cerium (Ce) was synthesized by sol-gel method. The structural and morphological properties were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDAX). The average crystallite size of the synthesized samples was determined as 20 nm. The functional group has been identified by FTIR. Antibacterial and anti-inflammatory focused the antibacterial activity and albumin denaturation of cerium. Blood compatibility was performed by hemolytic assay. Result The structural property was characterized by X-ray diffraction and crystallite size was determined as 20 nm. The FTIR spectra confirmed the presence of hydroxyl and phosphate functional groups. Antibacterial studies revealed that all cerium-doped hydroxyapatite samples exhibit good antibacterial activity against the pathogensStreptococcus oralisandStreptococcus pyogenes. Hemolysis ratios of the cerium-doped hydroxyapatite sample indicate good blood compatibility. Anti-inflammatory results exhibit high inhibition percentage of albumin denaturation for cerium-doped hydroxyapatite samples. Conclusion This study suggests that the cerium proves that it is a good material for biomedical applications.

Automated summary

The study focused on synthesizing and characterizing cerium-doped hydroxyapatite for biomedical applications. The cerium-doped samples showed good antibacterial activity and blood compatibility, as well as anti-inflammatory properties. Structural properties and functional groups were identified, with a crystallite size of 20 nm.