期刊
CANCER DISCOVERY
卷 10, 期 11, 页码 1742-1757出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/2159-8290.CD-20-0026
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类别
资金
- Incyte
- Constellation
- Prelude Therapeutics
- German research foundation [PA 2541/1-1]
- Memorial Sloan Kettering Cancer Center Support Grant [NIH P30 CA008748]
- National Cancer Institute [K99 CA248460, R35 CA197594-01A1, P01 CA108671 11]
- Sohn Foundation Fellowship of the Damon Runyon Cancer Research Foundation [DRG 22-17]
- StemLine Therapeutics
- StemLine
We investigated the role of PRMT5 in myeloproliferative neoplasm (MPN) pathogenesis and aimed to elucidate key PRMTS targets contributing to MPN maintenance. PRMTS is overexpressed in primary MPN cells, and PRMT5 inhibition potently reduced MPN cell proliferation ex vivo. PRMT5 inhibition was efficacious at reversing elevated hematocrit, leukocytosis, and splenomegaly in a model of JAK2(V)(617)(F+) polycythemia vera and leukocyte and platelet counts, hepatosplenomegaly, and fibrosis in the MPLW515L model of myelofibrosis. Dual targeting of JAK and PRMT5 was superior to JAK or PRMTS inhibitor monotherapy, further decreasing elevated counts and extramedullary hematopoiesis in vivo. PRMT5 inhibition reduced expression of E2F targets and altered the methylation status of E2F1 leading to attenuated DNA damage repair, cell-cycle arrest, and increased apoptosis. Our data link PRMTS to E2F1 regulatory function and MPN cell survival and provide a strong mechanistic rationale for clinical trials of PRMT5 inhibitors in MPN. SIGNIFICANCE: Expression of PRMT5 and E2F targets is increased in JAK2(V)(617)(F+) MPN. Pharmacologic inhibition of PRMT5 alters the methylation status of E2F1 and shows efficacy in JAK2(V)(617)(F)/MPLW515L MPN models and primary samples. PRMT5 represents a potential novel therapeutic target for MPN, which is now being clinically evaluated.
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