期刊
ACS CATALYSIS
卷 10, 期 17, 页码 9741-9746出版社
AMER CHEMICAL SOC
DOI: 10.1021/acscatal.0c03033
关键词
CODH; ACS; Wood-Ljungdahl; acetogenesis; metalloenzymes; carbon monoxide; acetyl coenzyme A; X-ray crystallography
资金
- NIH [T32 GM008334, R35 GM126982, R37 GM039451]
- Martin Family Society Fellowship for Sustainability
- National Institute of General Medical Sciences from the NIH [P30 GM124165]
- NIH Office of Research Infrastructure Programs High End Instrumentation grant [S10 RR029205]
- DOE Office of Science [DE-AC0206CH11357]
The Wood-Ljungdahl pathway allows for autotrophic bacterial growth on carbon dioxide, with the last step in acetyl-CoA synthesis catalyzed by the bifunctional enzyme carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS). ACS uses a complex Ni-Fe-S metallocluster termed the A-cluster to assemble acetyl-CoA from carbon monoxide, a methyl moiety and coenzyme A. Here, we report the crystal structure of CODH/ACS from Moorella thermoacetica with substrate carbon monoxide bound at the A-cluster, a state previously uncharacterized by crystallography. Direct structural characterization of this state highlights the role of second sphere residues and conformational dynamics in acetyl-CoA assembly, the biological equivalent of the Monsanto process.
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