期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-020-17311-4
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资金
- National Science Foundation [MCB-1412901]
- U.S. Department of Energy [DE-SC0001295]
- Agriculture and Food Research Initiative (AFRI) from the USDA National Institute of Food and Agriculture [2016-67013-24429, 1007600]
- U.S. Department of Energy (DOE) [DE-SC0001295] Funding Source: U.S. Department of Energy (DOE)
- NIFA [2016-67013-24429, 810722] Funding Source: Federal RePORTER
Various stress conditions induce the nuclear translocation of cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), but its nuclear function in plant stress responses remains elusive. Here we show that GAPC interacts with a transcription factor to promote the expression of heat-inducible genes and heat tolerance in Arabidopsis. GAPC accumulates in the nucleus under heat stress. Overexpression of GAPC enhances heat tolerance of seedlings and the expression of heat-inducible genes whereas knockout of GAPCs has opposite effects. Screening of Arabidopsis transcription factors identifies nuclear factor Y subunit C10 (NF-YC10) as a GAPC-binding protein. The effects of GAPC overexpression are abolished when NF-YC10 is deficient, the heat-induced nuclear accumulation of GAPC is suppressed, or the GAPC-NF-YC10 interaction is disrupted. GAPC overexpression also enhances the binding ability of NF-YC10 to its target promoter. The results reveal a cellular and molecular mechanism for the nuclear moonlighting of a glycolytic enzyme in plant response to environmental changes. Stress conditions can induce translocation of glyceraldehyde-3-phosphate dehydrogenase (GAPC) to the nucleus. Here Kim et al. show that in Arabidopsis, GAPC can interact with the NF-YC transcription factor subunit, enhance expression of heat-inducible genes and promote heat tolerance.
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