期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-020-16333-2
关键词
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资金
- Ministerio de Econom'a y Competitividad-MINECO [MAT2017-83111R, MAT2016-75362-C3-1-R]
- Comunidad de Madrid [B2017/BMD-3867]
- European Structural and Investment Fund
- UCM-Santander [CT17/17-CT18/17]
- China Scholarship Council (CSC) [201806870023]
- European Commission Horizon 2020 project NanoTBTech
- Fundacion para la Investigacion Biomedica del Hospital Universitario Ramon y Cajal [IMP18_38 (2018/0265)]
- UK Engineering and Physical Sciences Research Council [EP/M015130/1]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [431736/2018-9, 304967/20181]
- FINEP (Financiadora de Estudos e Projetos) [INFRAPESQ-11, INFRAPESQ-12]
- FAPEAL (Fundacao de Amparo a Pesquisa do Estado de Alagoas) [1209/2016]
- CNPq
- sandwich doctoral program (PDSE-CAPES) [88881/2016-01]
Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10mWcm(-2)) and doses (<0.5mgkg(-1)), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.
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