期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 36, 期 8, 页码 -出版社
SPRINGER
DOI: 10.1007/s11274-020-02891-6
关键词
Biofilm; Eradication; Gentamicin; Non-thermal plasma; Pseudomonas aeruginosa; Ti-6Al-4V
资金
- Operational Programme Prague-Competitiveness [CZ.2.16/3.1.00/24503]
- National Programme of Sustainability I-NPU I [LO1601]
- Charles University [Progress Q25]
Non-thermal plasma (NTP), generated at atmospheric pressure by DC cometary discharge with a metallic grid, and antibiotics (gentamicin-GTM, ceftazidime-CFZ and polymyxin B-PMB), either alone or in combination, were used to eradicate the mature biofilm of Pseudomonas aeruginosa formed on Ti-6Al-4V alloy. Our aim was to find the conditions for NTP pre-treatment capable of enhancing the action of the antibiotics and thus reducing their effective concentrations. The NTP treatment increased the efficacy of relatively low concentrations of antibiotics. Generally, the highest effect was achieved with GTM, which was able to suppress the metabolic activity of pre-formed P. aeruginosa biofilms in the concentration range of 4-9 mg/L by up to 99%. In addition, an apparent decrease of biofilm-covered area was confirmed after combined NTP treatment and GTM action by SYTO (R) 13 staining using fluorescence microscopy. Scanning electron microscopy confirmed a complete eradication of P. aeruginosa ATCC 15442 mature biofilm from Ti-6Al-4V alloy when using 0.25 h NTP treatment and subsequent treatment by 8.5 mg/L GTM. Therefore, NTP may be used as a suitable antibiofilm agent in combination with antibiotics for the treatment of biofilm-associated infections caused by this pathogen.
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