期刊
VETERINARY PARASITOLOGY
卷 297, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2020.109160
关键词
Trichinella spiralis; Cathepsin X; Invasion; Intestinal epithelial cells (IECs); Interaction
资金
- National Natural Science Foundation of China [U1704284]
TsCX, a crucial protease in Trichinella spiralis, promotes larval invasion of intestinal epithelial cells and is located throughout the worm body, potentially serving as a target for vaccines against enteral stages of the parasite.
The aim of this study was to ascertain the characteristics of a Trichinella spiralis cathepsin X (TsCX) and its role on larval invasion of intestinal epithelial cells (IECs). The full-length of TsCX cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of RT-PCR, IFA and Western blot revealed that TsCX was expressed at T. spiralis muscle larvae (ML), intestinal infective larvae, adult worm and newborn larvae, and it was located in whole worm section. The results of Far western and confocal microscopy demonstrated that there was a specific binding of rTsCX and IEC, and the binding site was located within the IEC cytoplasm. rTsCX promoted T. spiralis larval invasion of mouse IECs while anti-rTsCX antibody inhibited larval invasion into the IECs. Silencing TsCX by specific siRNA reduced the TsCX expression and larval invasive capacity. These results indicated that TsCX specifically binds to IECs and promotes larval invasion of intestinal epithelia, and it might be a potential target of vaccines against enteral stages of T. spiralis.
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