期刊
TOXICOLOGY LETTERS
卷 327, 期 -, 页码 58-68出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2020.03.016
关键词
Comet assay; Enzyme-modified comet assay; In vivo; Formamidopyrimidine DNA glycosylase; 8-Oxoguanine DNA glycosylase; Endonuclease III
类别
资金
- BIOGENSA project of the Ministry of Science, Innovation and Universities of the Spanish Government [AGL201570640-R]
- European Regional Development Fund (ERDF)
- Ministry of Science, Innovation and Universities of the Spanish Government [RYC-2013-14370]
- hCOMET project (COST Action) [CA 15132]
The in vivo comet assay is an established genotoxicity test, with an OECD test guideline, but in its standard form it measures only DNA strand breaks. Including in the assay an additional step, in which the DNA is incubated with a lesion-specific enzyme, can provide important information about the nature of the DNA damage. Formamidopyrimidine DNA glycosylase, 8-oxoguanine DNA glycosylase or endonuclease III are commonly used in the in vitro genotoxicity test and in human biomonitoring to detect oxidised bases, but in vivo applications are rarer. A systematic literature search has identified a total of 60 papers that report such in vivo experiments, testing a variety of agents. In many cases, strand breaks were not seen, but significant levels of enzyme-sensitive sites were induced - indicating a mechanism of action involving oxidative stress. Compounds such as methyl methanesulfonate (MMS) or ethyl methanesulfonate (EMS) could be used as positive controls in both the standard and the enzyme-modified in vivo comet assays.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据