Collagen Membrane for Guided Bone Regeneration in Dental and Orthopedic Applications

Treatment of cortical bone defects is a clinical challenge. Guided bone regeneration (GBR), commonly used in oral and maxillofacial dental surgery, may show promise for orthopedic applications in repair of cortical bone defects. However, a limitation in the use of GBR for cortical bone defects is the lack of an ideal scaffold that provides sufficient mechanical support to bridge the cortical bone with minimal interference in the repair process. We have developed a new collagen membrane, CelGro (TM), for use in GBR. We report the material characterization of CelGro and evaluate the performance of CelGro in translational preclinical and clinical studies. The results show CelGro has a bilayer structure of different fiber alignment and is composed almost exclusively of type I collagen. CelGro was found to be completely acellular and free from xenoantigen, alpha-gal (galactose-alpha-1,3-galactose). In the preclinical study of a rabbit cortical bone defect model, CelGro demonstrated enhanced bone-remodeling activity and cortical bone healing. Microcomputed tomography evaluation showed early bony bridging over the defect area 30 days postoperatively, and nearly complete restoration of mature cortical bone at the bone defect site 60 days postoperatively. Histological analysis 60 days after surgery further confirmed that CelGro enables bridging of the cortical bone defect by induction of newly formed cortical bone. Compared to a commercially available collagen membrane, Bio-Gide(R), CelGro showed much better cortical alignment and reduced porosity at the defect interface. As selection of orthopedic patients with cortical bone defects is complex, we conducted a clinical study evaluating the performance of CelGro in guided bone regeneration around dental implants. CelGro was used in GBR procedures in a total of 16 implants placed in 10 participants. Cone-beam computed tomography images show significantly increased bone formation both horizontally and vertically, which provides sufficient support to stabilize implants within 4 months. Together, the findings of our study demonstrate that CelGro is an ideal membrane for GBR not only in oral and maxillofacial reconstructive surgery but also in orthopedic applications (Clinical Trial ID ACTRN12615000027516). Impact statement Treatment of bone defects is a clinical challenge. In this study, we report the clinical translation of a type I collagen membrane for guided bone regeneration (GBR). The collagen membrane acts in an osteoconductive manner to facilitate cortical bone regeneration, and significantly improve cortical bone repair in the preclinical animal study. Together with the results from the clinical trial, showing satisfactory safety and clinical efficacy, our study indicates that CelGro collagen membrane can be used for GBR in both dental and orthopedic applications.

Automated summary

Treatment of cortical bone defects is a clinical challenge. In this study, a new type I collagen membrane for guided bone regeneration (GBR) has been clinically translated. The collagen membrane acts in an osteoconductive manner to facilitate cortical bone regeneration and improve cortical bone repair significantly, both in preclinical animal study and clinical trial. CelGro collagen membrane shows promise for GBR in both dental and orthopedic applications.